Semmelweis University. Website
Faculty of General Medicine. Department of Genetics, Cell- and Immunobiology. Budapest, Nagyvárad tér 4. 1089, Hungary
extracellular vesicles in autoimmunity, inflammation and in cardiovascular diseases
The PI and her group have previous experience in T cell immunity, autoimmunity, human and murine arthritides, and mast cell biology and redox regulation of immune cells. More than 10 years ago the group has also started to work on the isolation and characterization of extracellular vesicles (EVs). The group demonstrated for the first time that these subcellular structures share several biophysical parameters with protein aggregates, and protein aggregates may both interfere with extracellular vesicle detection by flow cytometry and contaminate vesicle preparations. They introduced the use of differential detergent lysis to prove the vesicular nature of particles and to distinguish EV subpopulations. They introduced the use of ACD blood sampling tube to prevent artefactual in vitro platelet vesiculation. They identified a disease-specific EV signature in the synovial fluid of patients with rheumatoid arthritis. The group has shown combinatorial effect of EVs with cytokines on the gene expression of cells. The group has first demonstrated the association of various glycosidases with EVs, and demonstrated that LDL mimics blood plasma-derived EVs during isolation and detection. Current research projects of the group focus on EV heterogeneity, the EV surface interactome, and the role of EVs in immunity and cardiovascular diseases.
- Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection. Sódar BW, Kittel Á, Pálóczi K, Vukman KV, Osteikoetxea X, Szabó-Taylor K, Németh A, Sperlágh B, Baranyai T, Giricz Z, Wiener Z, Turiák L, Drahos L, Pállinger É, Vékey K, Ferdinandy P, Falus A, Buzás EI. Sci Rep. 2016 Apr 18;6:24316
- Critical role of extracellular vesicles in modulating the cellular effects of cytokines. Szabó GT, Tarr B, Pálóczi K, Éder K, Lajkó E, Kittel Á, Tóth S, György B, Pásztói M, Németh A, Osteikoetxea X, Pállinger É, Falus A, Szabó-Taylor K, Buzás EI. Cell Mol Life Sci. 2014 Oct;71(20):4055-67.
- Emerging role of extracellular vesicles in inflammatory diseases. Buzas EI, György B, Nagy G, Falus A, Gay S. Nat Rev Rheumatol. 2014 Jun;10(6):356-64.
- Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. György B, Módos K, Pállinger E, Pálóczi K, Pásztói M, Misják P, Deli MA, Sipos A, Szalai A, Voszka I, Polgár A, Tóth K, Csete M, Nagy G, Gay S, Falus A, Kittel A, Buzás EI. Blood. 2011 Jan 27;117(4):e39-48.
- Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles. György B, Szabó TG, Pásztói M, Pál Z, Misják P, Aradi B, László V, Pállinger E, Pap E, Kittel A, Nagy G, Falus A, Buzás EI. Cell Mol Life Sci. 2011 Aug;68(16):2667-88